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FluorCam移動便攜式葉綠素?zé)晒獬上裣到y(tǒng)應(yīng)用于草地原位研究
發(fā)布時間: 2023-05-09 點(diǎn)擊次數(shù): 960次FluorCam移動便攜式葉綠素?zé)晒獬上?/span>是一個輕巧的可移動式便攜系統(tǒng),配備專用電池以及三腳架,適用于在野外和室內(nèi)對葉片和植物、生物結(jié)皮等進(jìn)行葉綠素?zé)晒鈩恿W(xué)分析
對于貼地植物和生物結(jié)皮等,成像系統(tǒng)自帶暗適應(yīng)室,可直接原位遮光進(jìn)行暗適應(yīng)及測量,成像面積31.5mm×41.5 mm,適合成片、單株或者單個葉片成像。對于株高較高或葉片較大的植物,還配備了暗適應(yīng)葉夾及更多暗適應(yīng)室方案(可聯(lián)系易科泰咨詢)
功能特點(diǎn):
· 可自己編輯測量實(shí)驗(yàn)程序(protocol)
· 具備自動重復(fù)測量功能
· 既能進(jìn)行持續(xù)光化學(xué)光成像測量,又可進(jìn)行PAM成像測量
· 高分辨率鏡頭,1360×1024像素,在最高圖像分辨率下時間分辨率可達(dá)每秒20幀,具備快照模式、動態(tài)視頻模式(binning模式)
· 多色矩陣光源:測量光620nm,飽和脈沖光可選白光、紅光或藍(lán)光,光化學(xué)光可選白光、紅光或藍(lán)光,4塊超亮LED光源板,每個光源板由5×5 LEDs陣列,尺寸4×4 cm
· 完備的自動測量程序,可自由對測量程序進(jìn)行編輯
Fv/Fm:測量參數(shù)包括Fo,Fm,Fv,QY等
Kautsky誘導(dǎo)效應(yīng):Fo,Fp,Fv,Ft_Lss,QY,Rfd等熒光參數(shù)
熒光淬滅分析:Fo,Fm,Fp,Fs,Fv,QY,ΦII,NPQ,Qp,Rfd,qL等50多個參數(shù)
光響應(yīng)曲線LC:Fo,Fm,QY,QY_Ln,ETR等熒光參數(shù)
獲得的所有參數(shù)都有數(shù)值及成像圖,并且均可保存、導(dǎo)出。
· 成像預(yù)處理:程序軟件可自動識別多個植物樣品或多個區(qū)域,也可手動選擇區(qū)域(Region of interest,ROI)。手動選區(qū)的形狀可以是方形、圓形、任意多邊形或扇形。軟件可自動測量分析每個樣品和選定區(qū)域的熒光動力學(xué)曲線及相應(yīng)參數(shù),樣品或區(qū)域數(shù)量不受限制(>1000)
測量實(shí)例:
參考文獻(xiàn):
1. Shomali A , Aliniaeifard S , Didaran F , et al. Synergistic Effects of Melatonin and Gamma-Aminobutyric Acid on Protection of Photosynthesis System in Response to Multiple Abiotic Stressors[J]. Cells, 2021, 10(7):1631.
2. Rao L, Li S, Cui X. Leaf morphology and chlorophyll fluorescence characteristics of mulberry seedlings under waterlogging stress[J]. Scientific Reports, 2021, 11(1): 1-11.
3. Rane J, Sharma D, Ekatpure S, et al. Relative tolerance of photosystem II in spike, leaf, and stem of bread and durum wheat under desiccation[J]. Photosynthetica, 2019, 57(4): 1100-1108.
4. Giudici G N M. Combined chlorophyll fluorescence techniques to study environmental impact on the mountain moss Polytrichum commune[J]. Czech Polar Reports, 2021, 11(1): 161-173.
5. Jung J Y, Min C W, Kim S W, et al. Proteomic Analysis of Ginseng (Panax ginseng CA Meyer) Fluid Proteins under Salt Stress[J]. Agronomy, 2022, 12(9): 2048.
6. De Agostini A, Cogoni A, Cortis P, et al. Heavy metal tolerance strategies in metallicolous and non-metallicolous populations of mosses: Insights of γ+ β-tocopherol regulatory role[J]. Environmental and Experimental Botany, 2022, 194: 104738.